Journal: Biological Research
Article Title: Unveiling a novel memory center in human brain: neurochemical identification of the nucleus incertus , a key pontine locus implicated in stress and neuropathology
doi: 10.1186/s40659-024-00523-z
Figure Lengend Snippet: In situ hybridization of RLN3 mRNA in the NI. A RLN3 mRNA detection in coronal anterior hemi-pons of the human brain. B Higher magnification images of RLN3 mRNA (green particles) and C vGAT1 mRNA (yellow particles) co-localized in neurons in the NI area (merge). Inset represents the hemi-pons section under brightfield illumination. Abbreviations: 4V, fourth ventricle, mlf, medial longitudinal fasciculus, NI, nucleus incertus , vGAT1 , vesicular GABA transporter-1
Article Snippet: Imaging of tissue sections stained using immunohistochemistry was performed using an Olympus IX70 microscope equipped with brightfield illumination (Olympus, Tokyo, Japan).
Techniques: In Situ Hybridization